Sıçanlarda Lipopolisakkarit ile Oluşturulan Üveit Üzerine Nar Çekirdeği Yağının Etkileri


Özet Görüntüleme: 207 / PDF İndirme: 105

Yazarlar

  • Tugba Nurcan YUKSEL Tekirdağ Namık Kemal Univercity
  • Muhammed YAYLA
  • Erdem TOKTAY
  • Duygu KOSE
  • Zekai HALICI

DOI:

https://doi.org/10.5281/zenodo.8205274

Anahtar Kelimeler:

Anti-inflamatuvar, Antioksidant, Nar Çekirdeği Yağı, Punikalagin, Üveit

Özet

Amaç: Nar çekirdeği yağı (NÇY), punikalagin gibi antioksidan ve antiinflamatuar özelliklere sahip fenolik bileşikler içerir. Bu çalışmada deneysel lipopolisakkarit (LPS) kaynaklı üveit modelinde NÇY’nin koruyucu etkilerini değerlendirmeyi amaçladık.

Yöntem: 50 Wistar Albino sıçan rastgele beş gruba ayrıldı. Her grup 3. ve 24. saat olmak üzere iki alt gruba ayrıldı (n=5). Sağlıklı grup intraperitoneal normal salin, üveit grubu subkutan 200 μg/kg LPS, dexamethasone grubu 200μg/kg LPS ile birlikte intraperitoneal 1mg/kg deksametazon, NÇY 0.5 grubu 200μg/kg LPS ile birlikte 0,5 mg/kg NÇY ve NÇY 1 grubu 200μg/kg LPS ile birlikte 1 mg/kg NÇY aldı.. Deneyin sonunda göz dokuları toplandı ve biyokimyasal (Glutatyon, Süperoksit distmütaz ve Malondialdehit seviyeleri) ve histopatolojik (Harris Hematoksilen ve Eosin Y ile boyama) olarak incelendi.

Bulgular: NÇY uygulaması, üveitin sebep olduğu oksidatif stres faktörlerindeki değişiklikleri önemli ölçüde iyileştirdi. NÇY uygulaması üveit grubuna kıyasla göz dokularında inflamasyon bulguları dahil olmak üzere histopatolojik değişiklikleri ortadan kaldırdı. Ancak, tüm bu sonuçlar sağlıklı grubunki kadar güçlü değildi.

Sonuç: Üveite karşı koruyucu etkilerini belirlemek için gelecekte nar çekirdeği yağının farklı dozları ile çalışmalar yapılabilir. Belki de NÇY, doğal içeriğinin yanısıra antioksidan ve antienflamatuar etkileri ile üveit için doğal koruyucu ilaçların üretilmesinde önemli bir rol oynayabilir.

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2023-08-02

Nasıl Atıf Yapılır

YUKSEL , T. N., YAYLA , M., TOKTAY , E., KOSE , D., & HALICI, Z. (2023). Sıçanlarda Lipopolisakkarit ile Oluşturulan Üveit Üzerine Nar Çekirdeği Yağının Etkileri. GEVHER NESİBE TIP VE SAĞLIK BİLİMLERİ DERGİSİ, 8(3), 504–512. https://doi.org/10.5281/zenodo.8205274

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Cilt 8 Sayı 3 (2023)

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