Aerobic ve Anaerobic Yetişkin Kan Kültürü Şişelerinde Izolatlarin Üremesi ve Üreme Sürelerinin Değerlendirilmesi
Özet Görüntüleme: 209 / PDF İndirme: 130
DOI:
https://doi.org/10.5281/zenodo.7133100%20Anahtar Kelimeler:
Kan kültürü, Üreme zamanı, Aerobik ve Anaerobik, Koagülaz negatif stafilokoklar, MayaÖzet
Bu çalışmada dolaşım sistemi enfeksiyonlarının en kısa sürede tespitinde aerobik ve anaerobik kan kültürü şişelerinin birlikte kullanılmasının ve alınan kan hacminin etkisinin yorumlanması amaçlandı. Kan kültürleri, standart mikrobiyolojik yöntemlerin yanı sıra BD BACTEC 9240 (Becton Dickinson, ABD) kullanılarak belirlendi. İzolatların aerobik ve anaerobik kan kültürü şişelerinde büyüme ve büyüme süreleri karşılaştırıldı ve ölçüldü. Toplam 11234 kan kültürü şişesinden 8178'i değerlendirildi. 974 (%11.9) kan kültüründe mikrobiyal üreme saptandı. Etken ajan olarak kabul edilen başlıca patojenler koagülaz negatif stafilokoklar 114 (%18), S. aureus 108 (%17,1), Klebsiella spp 86 (%13,6), E. coli 63 (%9,9), maya 45 (%7,1). ) ve Acinetobacter spp 43 (%6,8) tespit edildi. Kan kültürlerinde klinik olarak anlamlı büyüme oranı %6.3; yanlış pozitif ve negatif oranları sırasıyla %0.2, %0.06 olarak görüldü. Kan kültürü şişelerinde büyütülen klinik olarak anlamlı izolatların %11'inde sadece anaerobik şişede üreme gözlendi. Kullanılan kan kültürü şişelerinden Pseudomonas sp, acinetobacter spp ve mantar üremelerinin büyük kısmı anaerobik şişeye kıyasla aerobik şişede görülmüştür (P <0.05 ). Aerobik ve anaerobik şişelerin ortalama sinyal süreleri sırasıyla 18.5 ve 20.9 saat olarak gözlendi. Tüm bulgular sonucunda Aerobik ve anaerobik kan kültürü şişelerinin birlikte kullanılmasının ve alınan kan hacminin kan dolaşımı enfeksiyonlarının hızlı tespitinde çok değerli olduğu sonucuna varıldı.
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