Protective Effects of Montelucast and N-Acetlycystein Activity on Doxorubicin-Induced Liver Damage in Animal Model: Paraoxonase and Arylesterase
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https://doi.org/10.5281/zenodo.11373515Keywords:
oxidative stress, Doxorubicin, N-Acetylcysteine, Montelukast, LiverAbstract
Objective: Doxorubicin (DOX) is a chemotherapeutic agent and is widely used in cancer treatment. There are some studies suggesting oxidative stress-induced toxic changes in the liver due to DOX administration. The aim of this study was to reveal the oxidative damage of DOX in liver tissue at molecular level and to evaluate the protective effect of N-acetyl cysteine and Montelukast against DOX oxidative damage.
Methods: Thirty-six male rats were equally divided into 6 groups. The first group was used as control. The second group received a single dose of DOX. The third and fourth groups received N-Acetylcysteine and Montelukast respectively for 28 days. The fifth and sixth groups received a single dose of DOX, followed by N-Acetylcysteine and Montelukast for 28 days. NAC was given for 10 days. At the end of the experiment, liver tissues were taken from all animals under anesthesia. Malondialdehyde (MDA), Paraoxanase and Arylesterase levels were determined in these samples by spectrophotometric methods.
Results: It was determined that MDA level increased, paraoxanase and arylesterase levels decreased in the group given Doxo compared to the control group. In addition, MDA and Paraoxanase levels increased in the Doxorubicin+N-Acetylsitein group, but did not approach the control group levels.
Conclusion: It was concluded that Doxorubicin administration increased oxidative stress and NAC administration could prevent the increased oxidative stress. NAC caused modulatory effects on oxidative stress and antioxidant redox system in Doxorubicin-induced liver toxicity in the rat.
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