The Effects on Pomegranate Seed Oil in Lipopolysaccharide - Induced Uveitis in Rats
Abstract views: 201
/ 
PDF downloads: 103
DOI:
https://doi.org/10.5281/zenodo.8205274Keywords:
Anti-Inflammatory, , Anti-Oxidant, Pomegranate Seed Oil, Punicalagin, UveitisAbstract
Purpose: Pomegranate seed oil (PSO) contains antioxidant and anti-inflammatory phenolic compounds such as punicalagin. We aimed to determine the protective effects of PSO on lipopolysaccharide-induced uveitis in rats.
Method: 50 Wistar Albino rats were randomly divided into five groups. Each group had two subgroups: the 3rd and 24th hour (n=5). Group healthy received intraperitoneal normal saline, group uveitis received subcutaneous 200 μg/kg lipopolysaccharide (LPS), group dexamethasone received 200μg/kg LPS plus 1mg/kg dexamethasone intraperitoneal, group PSO 0.5 received 200μg/kg LPS plus 0.5 mg/kg PSO, and Group PSO 1 received 200μg/kg LPS plus 1 mg/kg PSO. Each group had two subgroups: the 3rd and 24th hour. At the end of the experiment, eye tissues were removed for biochemically (Glutathione, Superoxide dismutase and Malondialdehyde levels) and histopathologically (staining with Harris Hematoxylin and Eosin Y) investigation.
Results: PSO administration significantly ameliorated modifications in uveitis-induced oxidative stress factors. PSO administration eliminated histopathological changes in the eye tissues, including inflammatory findings, in comparison to the uveitis group. However, all these results were not as strong as those of the healthy group.
Conclusion: Future studies may be conducted with different doses of pomegranate seed oil to determine its protective effects against uveitis. Perhaps, PSO, with its antioxidant and anti-inflammatory effects, in addition to its natural content, may play an important role in the production of natural preventive drugs for uveitis.
References
attenuates renal ischemia/reperfusion injury in rats through suppression of inflammation, oxidative stress and apoptosis. Biomed Pharmacother, 110, 9-19. doi:10.1016/j.biopha.2018.11.045
Adams, L. S., Seeram, N. P., Aggarwal, B. B., Takada, Y., Sand, D., & Heber, D. (2006). Pomegranate juice, total pomegranate ellagitannins, and punicalagin suppress inflammatory cell signaling in colon cancer cells. J Agric Food Chem, 54(3), 980-985. doi:10.1021/jf052005r
Aviram, M., & Rosenblat, M. (2012). Pomegranate Protection against Cardiovascular Diseases. Evid Based Complement Alternat Med, 2012, 382763. doi:10.1155/2012/382763
Bachagol, D., Joseph, G. S., Ellur, G., Patel, K., Aruna, P., Mittal, M., . . . Sharan, K. (2018). Stimulation of liver IGF-1 expression promotes peak bone mass achievement in growing rats: a study with pomegranate seed oil. Journal of Nutritional Biochemistry, 52, 18-26. doi:10.1016/j.jnutbio.2017.09.023
Bialek, A., Bialek, M., Lepionka, T., Pachniewicz, P., & Czauderna, M. (2021). Oxysterols and lipidomic profile of myocardium of rats supplemented with pomegranate seed oil and/or bitter melon aqueous extract - Cardio-oncological animal model research. Chem Phys Lipids, 235, 105057. doi:10.1016/j.chemphyslip.2021.105057
Boroushaki, M. T., Mollazadeh, H., & Afshari, A. R. (2016). Pomegranate seed oil: A comprehensive review on its therapeutic effects. Int J Pharm Sci Res, 7(2), 430.
Boussetta, T., Raad, H., Letteron, P., Gougerot-Pocidalo, M. A., Marie, J. C., Driss, F., & El-Benna, J. (2009). Punicic acid a conjugated linolenic acid inhibits TNFalpha-induced neutrophil hyperactivation and protects from experimental colon inflammation in rats. PLoS One, 4(7), e6458. doi:10.1371/journal.pone.0006458
Chang, Y. H., Horng, C. T., Chen, Y. H., Chen, P. L., Chen, C. L., Liang, C. M., . . . Chen, J. T. (2008). Inhibitory effects of glucosamine on endotoxin-induced uveitis in Lewis rats. Invest Ophthalmol Vis Sci, 49(12), 5441-5449. doi:10.1167/iovs.08-1784
Chesnokova, N. B., Neroev, V. V., Beznos, O. V., Beishenova, G. A., Nikol'skaia, II, Kost, O. A., . . . Shekhter, A. B. (2014). [Oxidative stress in uveitis and its correction with superoxide dismutase antioxidative enzyme (experimental study)]. Vestn Oftalmol, 130(5), 30-34. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/25711059
Curnow, S. J., & Murray, P. I. (2006). Inflammatory mediators of uveitis: cytokines and chemokines. Current Opinion in Ophthalmology, 17(6), 532-537. doi:DOI 10.1097/ICU.0b013e32801094b5
Da Silva, J. A. T., Rana, T. S., Narzary, D., Verma, N., Meshram, D. T., & Ranade, S. A. (2013). Pomegranate biology and biotechnology: A review. Scientia Horticulturae, 160, 85-107. doi:10.1016/j.scienta.2013.05.017
Devos, A. F., Vanharen, M. A. C., Verhagen, C., Hoekzema, R., & Kijlstra, A. (1994). Kinetics of Intraocular Tumor-Necrosis-Factor and Interleukin-6 in Endotoxin-Induced Uveitis in the Rat. Investigative Ophthalmology & Visual Science, 35(3), 1100-1106. Retrieved from ://WOS:A1994MZ26100042
Dick, A. D., Tundia, N., Sorg, R., Zhao, C., Chao, J., Joshi, A., & Skup, M. (2016). Risk of Ocular Complications in Patients with Noninfectious Intermediate Uveitis, Posterior Uveitis, or Panuveitis. Ophthalmology, 123(3), 655-662. doi:10.1016/j.ophtha.2015.10.028
Dunn, J. P. (2015). Uveitis. Prim Care, 42(3), 305-323. doi:10.1016/j.pop.2015.05.003
Durrani, O. M., Tehrani, N. N., Marr, J. E., Moradi, P., Stavrou, P., & Murray, P. I. (2004). Degree, duration, and causes of visual loss in uveitis. Br J Ophthalmol, 88(9), 1159-1162. doi:10.1136/bjo.2003.037226
Gil, M. I., Tomas-Barberan, F. A., Hess-Pierce, B., Holcroft, D. M., & Kader, A. A. (2000). Antioxidant activity of pomegranate juice and its relationship with phenolic composition and processing. Journal of Agricultural and Food Chemistry, 48(10), 4581-4589. doi:10.1021/jf000404a
Harzallah, A., Hammami, M., Kepczynska, M. A., Hislop, D. C., Arch, J. R., Cawthorne, M. A., & Zaibi, M. S. (2016). Comparison of potential preventive effects of pomegranate flower, peel and seed oil on insulin resistance and inflammation in high-fat and high-sucrose diet-induced obesity mice model. Arch Physiol Biochem, 122(2), 75-87. doi:10.3109/13813455.2016.1148053
Hosseini, A., Rajabian, A., Forouzanfar, F., Farzadnia, M., & Boroushaki, M. T. (2022). Pomegranate seed oil protects against tacrolimus-induced toxicity in the heart and kidney by modulation of oxidative stress in rats. Avicenna J Phytomed, 12(4), 439-448. doi:10.22038/AJP.2022.19703
Imrie, F. R., & Dick, A. D. (2007). Biologics in the treatment of uveitis. Curr Opin Ophthalmol, 18(6), 481-486. doi:10.1097/ICU.0b013e3282f03d42
Jabs, D. A., Nussenblatt, R. B., Rosenbaum, J. T., & Standardization of Uveitis Nomenclature Working, G. (2005). Standardization of uveitis nomenclature for reporting clinical data. Results of the First International Workshop. Am J Ophthalmol, 140(3), 509-516. doi:10.1016/j.ajo.2005.03.057
Jaffe, G. J., Dick, A. D., Brezin, A. P., Nguyen, Q. D., Thorne, J. E., Kestelyn, P., . . . Suhler, E. B. (2016). Adalimumab in Patients with Active Noninfectious Uveitis. N Engl J Med, 375(10), 932-943. doi:10.1056/NEJMoa1509852
Kaseke, T., Opara, U. L., & Fawole, O. A. (2020). Effect of Microwave Pretreatment of Seeds on the Quality and Antioxidant Capacity of Pomegranate Seed Oil. Foods, 9(9). doi:10.3390/foods9091287
Keles, S., Halici, Z., Atmaca, H. T., Yayla, M., Yildirim, K., Ekinci, M., . . . Bayraktutan, Z. (2014). The ocular endothelin system: a novel target for the treatment of endotoxin-induced uveitis with bosentan. Invest Ophthalmol Vis Sci, 55(6), 3517-3524. doi:10.1167/iovs.14-14193
Kim, E. Y., & Moudgil, K. D. (2008). Regulation of autoimmune inflammation by pro-inflammatory cytokines. Immunol Lett, 120(1-2), 1-5. doi:10.1016/j.imlet.2008.07.008
Lansky, E. P., & Newman, R. A. (2007). Punica granatum (pomegranate) and its potential for prevention and treatment of inflammation and cancer. Journal of Ethnopharmacology, 109(2), 177-206. doi:10.1016/j.jep.2006.09.006
Lowry, O. H., Rosebrough, N. J., Farr, A. L., & Randall, R. J. (1951). Protein measurement with the Folin phenol reagent. J Biol Chem, 193(1), 265-275. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/14907713
Lu, H., Chen, D. D., & Zhu, S. Q. (2022). Therapeutic potential of pupilloplasty combined with phacomulsification and intraocular lens implantation against uveitis-induced cataract. Int J Ophthalmol, 15(10), 1598-1603. doi:10.18240/ijo.2022.10.06
Merida, S., Sancho-Tello, M., Almansa, I., Desco, C., Peris, C., Moreno, M. L., . . . Bosch-Morell, F. (2018). Bevacizumab Diminishes Inflammation in an Acute Endotoxin-Induced Uveitis Model. Front Pharmacol, 9, 649. doi:10.3389/fphar.2018.00649
Palabiyik, S. S., Karakus, E., Akpinar, E., Halici, Z., Bayir, Y., Yayla, M., & Kose, D. (2016). The Role of Urotensin Receptors in the Paracetamol-Induced Hepatotoxicity Model in Mice: Ameliorative Potential of Urotensin II Antagonist. Basic Clin Pharmacol Toxicol, 118(2), 150-159. doi:10.1111/bcpt.12447
Patel, A. K., Newcomb, C. W., Liesegang, T. L., Pujari, S. S., Suhler, E. B., Thorne, J. E., . . . Systemic Immunosuppressive Therapy for Eye Diseases Research, G. (2016). Risk of Retinal Neovascularization in Cases of Uveitis. Ophthalmology, 123(3), 646-654. doi:10.1016/j.ophtha.2015.10.056
Planck, S. R., Huang, X. N., Robertson, J. E., & Rosenbaum, J. T. (1994). Cytokine Messenger-Rna Levels in Rat Ocular-Tissues after Systemic Endotoxin Treatment. Investigative Ophthalmology & Visual Science, 35(3), 924-930. Retrieved from ://WOS:A1994MZ26100021
Rosenbaum, J. T., McDevitt, H. O., Guss, R. B., & Egbert, P. R. (1980). Endotoxin-induced uveitis in rats as a model for human disease. Nature, 286(5773), 611-613. doi:10.1038/286611a0
Satici, A., Guzey, M., Gurler, B., Vural, H., & Gurkan, T. (2003). Malondialdehyde and antioxidant enzyme levels in the aqueous humor of rabbits in endotoxin-induced uveitis. Eur J Ophthalmol, 13(9-10), 779-783. doi:10.1177/1120672103013009-1008
Seeram, N. P., Adams, L. S., Henning, S. M., Niu, Y. T., Zhang, Y. J., Nair, M. G., & Heber, D. (2005). In vitro antiproliferative, apoptotic and antioxidant activities of punicalagin, ellagic acid and a total pomegranate tannin extract are enhanced in combination with other polyphenols as found in pomegranate juice. Journal of Nutritional Biochemistry, 16(6), 360-367. doi:10.1016/j.jnutbio.2005.01.006
Sen, H. N., Vitale, S., Gangaputra, S. S., Nussenblatt, R. B., Liesegang, T. L., Levy-Clarke, G. A., . . . Kempen, J. H. (2014). Periocular corticosteroid injections in uveitis: effects and complications. Ophthalmology, 121(11), 2275-2286. doi:10.1016/j.ophtha.2014.05.021
Seve, P., Cacoub, P., Bodaghi, B., Trad, S., Sellam, J., Bellocq, D., . . . Kodjikian, L. (2017). Uveitis: Diagnostic work-up. A literature review and recommendations from an expert committee. Autoimmun Rev, 16(12), 1254-1264. doi:10.1016/j.autrev.2017.10.010
Shaban, N. Z., Mohammed, A. S., Abu-Serie, M. M., Maher, A. M., & Habashy, N. H. (2022). Inhibition of oxidative stress, IL-13, and WNT/beta-catenin in ovalbumin-sensitized rats by a novel organogel of Punica granatum seed oil saponifiable fraction. Biomed Pharmacother, 154, 113667. doi:10.1016/j.biopha.2022.113667
Shi, L., Hager, T., Fries, F. N., Daas, L., Holbach, L., Hofmann-Rummelt, C., . . . Szentmary, N. (2019). Reactive uveitis, retinal vasculitis and scleritis as ocular end-stage of Acanthamoeba keratitis: a histological study. Int J Ophthalmol, 12(12), 1966-1971. doi:10.18240/ijo.2019.12.20
Shoeb, M., Zhang, M., Xiao, T. L., Syed, M. E., & Ansari, N. H. (2018). Amelioration of Endotoxin-Induced Inflammatory Toxic Response by a Metal Chelator in Rat Eyes. Investigative Ophthalmology & Visual Science, 59(1), 31-38. doi:10.1167/iovs.17-22172
Shrivas, M., Khunt, D., Shrivas, M., & Misra, M. (2023). Studies on pomegranate seed oil enriched galantamine hydrobromide microemulsion: formulation, in vitro antioxidant and neuroprotective potential. Pharm Dev Technol, 28(2), 153-163. doi:10.1080/10837450.2023.2171433
Sijssens, K. M., Rijkers, G. T., Rothova, A., Stilma, J. S., & de Boer, J. H. (2008). Distinct cytokine patterns in the aqueous humor of children, adolescents and adults with uveitis. Ocul Immunol Inflamm, 16(5), 211-216. doi:10.1080/09273940802409969
Smith, J. A., Mackensen, F., Sen, H. N., Leigh, J. F., Watkins, A. S., Pyatetsky, D., . . . Goldstein, D. A. (2009). Epidemiology and course of disease in childhood uveitis. Ophthalmology, 116(8), 1544-1551, 1551 e1541. doi:10.1016/j.ophtha.2009.05.002
Smith, J. R., Hart, P. H., & Williams, K. A. (1998). Basic pathogenic mechanisms operating in experimental models of acute anterior uveitis. Immunol Cell Biol, 76(6), 497-512. doi:10.1046/j.1440-1711.1998.00783.x
Straccia, M. C., Siano, F., Coppola, R., La Cara, F., & Volpe, M. (2012). Extraction and characterization of vegetable oils from cherry seed by different extraction processes. Chemical Engineerin g Transactions, 27, 391-396.
Tatar, A., Yayla, M., Kose, D., Halici, Z., Yoruk, O., & Polat, E. (2016). The role of endothelin-1 and endothelin receptor antagonists in allergic rhinitis inflammation: ovalbumin-induced rat model. Rhinology, 54(3), 266-272. doi:10.4193/Rhin15.059
Turk, A., Aykut, M., Akyol, N., Kola, M., Mentese, A., Sumer, A., . . . Erdol, H. (2014). Serum anti-carbonic anhydrase antibodies and oxidant-antioxidant balance in patients with acute anterior uveitis. Ocul Immunol Inflamm, 22(2), 127-132. doi:10.3109/09273948.2013.830753
Ugan, R. A., & Un, H. (2020). The Protective Roles of Butein on Indomethacin Induced Gastric Ulcer in Mice. Eurasian J Med, 52(3), 265-270. doi:10.5152/eurasianjmed.2020.20022
Viladomiu, M., Hontecillas, R., Lu, P., & Bassaganya-Riera, J. (2013). Preventive and prophylactic mechanisms of action of pomegranate bioactive constituents. Evid Based Complement Alternat Med, 2013, 789764. doi:10.1155/2013/789764
Xu, J., Guo, C. J., Yang, J. J., Wei, J. Y., Li, Y. F., Pang, W., . . . Cheng, S. (2005). [Intervention of antioxidant system function of aged rats by giving fruit juices with different antioxidant capacities]. Zhonghua Yu Fang Yi Xue Za Zhi, 39(2), 80-83. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/15842822
Yadav, U. C., Kalariya, N. M., & Ramana, K. V. (2011). Emerging role of antioxidants in the protection of uveitis complications. Curr Med Chem, 18(6), 931-942. doi:10.2174/092986711794927694
Yadav, U. C., Subramanyam, S., & Ramana, K. V. (2009). Prevention of endotoxin-induced uveitis in rats by benfotiamine, a lipophilic analogue of vitamin B1. Invest Ophthalmol Vis Sci, 50(5), 2276-2282. doi:10.1167/iovs.08-2816
Yadav, U. C. S., & Ramana, K. V. (2019). Endotoxin-Induced Uveitis in Rodents. Methods Mol Biol, 1960, 161-168. doi:10.1007/978-1-4939-9167-9_14
Yanai, R., Takeda, A., Yoshimura, T., & Sonoda, K. H. (2014). [Pathophysiology and new treatment of uveitis]. Nihon Rinsho Meneki Gakkai Kaishi, 37(2), 74-82. doi:10.2177/jsci.37.74
Yayla, M., Cetin, D., Adali, Y., Kilicle, P. A., & Toktay, E. (2018). Potential therapeutic effect of pomegranate seed oil on ovarian ischemia/reperfusion injury in rats. Iran J Basic Med Sci, 21(12), 1262-1268. doi:10.22038/ijbms.2018.30149.7268
Yuksel, T. N., Yayla, M., Kose, D., Halici, Z., Bozkurt, E., & Toktay, T. (2023). Protective effects of melatonin receptor agonists on endotoxin-induced uveitis in rats. Iran J Basic Med Sci, 26(5), 540-548. doi:10.22038/IJBMS.2023.67297.14749
Zhang, J., Zhao, F. F., Bai, S., Zheng, J., Zheng, P., & Dai, G. D. (2013). [Study on inhibitory effect of lycium pigment on lipopolysaccharide-induced uveitis in rats and its mechanism]. Zhongguo Zhong Yao Za Zhi, 38(11), 1778-1782. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/24010295
Zhang, N., Yu, S., Liu, X., & Lu, H. (2017). Low Dose of Lipopolysaccharide Pretreatment Preventing Subsequent Endotoxin-Induced Uveitis Is Associated with PI3K/AKT Pathway. J Immunol Res, 2017, 1273940. doi:10.1155/2017/1273940
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 GEVHER NESIBE JOURNAL OF MEDICAL AND HEALTH SCIENCES
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
The journal is licensed under a 