Evaluation Of The Connection Between Serum Tumor Necrosis Factor-Alpha, Transforming Growth Factor-Beta, Interleukin-10 And Alanine Aminotransferase Levels And Treatment In Naive Chronic Hepatitis C Patients

Abstract

Patients with naive CHC were included in the study. All of the patients were genotype 1b and all of them were treated with pegylated interferon and ribavirin combined. 43 people were included in the patient group and 43 people in the control group. Of the patients in both the patient and control groups, 23 were male and 20 were female. The mean age of the patient group was 49.54±13.02, and the mean age of the control group was 47.98±14.1. Serum alanine aminotransferase (ALT), TNF-alpha, TGF-beta, IL-10 values were studied in the patient and control groups. To measure TNF-alpha, TGF-beta and IL-10 values, 10 cc of venous blood was taken into biochemistry tubes containing paraffin. Blood was centrifuged at 5000 rpm for 5 minutes. These procedures were carried out within 1 hour. After centrifugation, the serums placed in the Eppendorf tube were stored in a deep freezer at -80 degrees until the study day. Serum cytokine levels were measured by ELISA method according to kit procedures. Levels of Human TNF-alpha (DIAsource TNF-alpha - ELISA Kit), Human IL-10 (DIAsource IL-10-ELISA Kit), Human TGF-beta ELISA-Multispecies TGF-beta kit) in the patient group at 0. 12. And it was studied from serum samples at week 48 and from a single serum sample in the control group. Values detected in serum samples were recorded as pg/ml. Serum ALT values in the patient and control groups were routinely studied in the biochemistry laboratory of our hospital. Combined treatment was started in the case group and the patients were evaluated in terms of response to the treatment at the 12th and 48th weeks. They were divided into 2 groups as responding and non-responsive to treatment. TNF-alpha, TGF-beta and IL-10 serum cytokine values of these two groups were 0-12 weeks, 12-48 weeks and 0-48. Pairwise comparisons were made within weeks. Finally, cytokine values at 0, 12, 48 weeks were compared with ALT. Spearman's correlation test was used for statistical analysis. The limit of significance was taken as p<0.05 in the interpretations. SPSS (version: 17.0) package program was used for statistical analysis. A statistical difference was found in serum TNF-alpha, TGF-beta and IL-10 serum cytokine values in patients who responded to treatment.