POSSIBLE PROTECTIVE EFFECT OF MELATONIN ON THE CORTI ORGAN AUTOXICITY

Abstract

Introduction: Endocrine disruptors or hormone-like chemicals in our environment compromise metabolism by suppressing the effects of endogenous hormones. One of them Nonylphenol (NFA) as a result of bioaccumulation in living tissues to form oxidative stress. This causes damage to lipids, proteins and DNA structure, cellular reactions and formation in the body. Melatonin released from the pineal gland at night has an important function in circadian rhythm and functions as a protector against oxidative stress caused by reactive oxygen species. Objective: To determine the possible protective effect of melatonin, which is antioxidant, against the autotoxicity that Nonylphenol will cause in the corti organ. Method: Wistar albino female rats were divided into 3 groups (Control group, Nonylphenol group, Nonylphenol + Melatonin group). No treatment was applied to the control group. Nonylphenol group was given by gavage by dissolving a single dose of 50μl Nonylphenol in 100μl corn oil on the 16th day. In the Nonylphenol + Melatonin group, 10mg / kg melatonin was injected intraperitoneally for 15 days, and on the 16th day, a single dose of 50μl Nonylphenol was dissolved in 100μl corn oil and administered via gavage. The following day, cocheal tissues were removed under anesthesia and fixed in 10% formaldehyde. After decalcified process step, following after routine tissue was stained with hematoxylin and eosin and Masson's Trichrome. Histopathological and morphometric evaluations were made in line with the previously determined criteria. Results: It was determined that the lengths of tectorial and basilar membranes in the basal, media and apex regions of the cochleae belonging to nonylphenol treated groups occurred but were not statistically significant (p> 0.05). It was determined that nonylphenol application affects the outer and inner hair cells located in the apex of the cochlea and the shortened cell lengths and this shortening was statistically significant (p <0.05). outer hair cells in the basal region of the cochlea there was significant difference in the level between all groups when evaluated statistically significant (p <0.05). It was also among the morphometric findings that nonylphenol application caused a narrowing of the width of the citria vascularis in all turns of the cochlea. Conclusion: on the cochlear application of Nonylphenol brought ototoxicity was determined to occur and also the protective effect of melatonin within antioxidant.