The Effect of MMP-2 (-1306 C/T) Gene Variation in the Development of Ischemic Stroke
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https://doi.org/10.5281/zenodo.7388026Keywords:
Ischemicstroke, MMP-2 gene, MMP-2 (-1306 C/T) gene variation, PCR, RFLPAbstract
The aim of this study is to determine the role of MMP-2 (-1306 C/T) gene variation in the development of ischemic stroke. Our study was carried out with 93 ischemic stroke patients and 94 healthy controls. Polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) methods were applied to determine the genotype distributions of MMP-2 (-1306 C/T) gene variation. Although the significant difference was not determined between ischemic stroke patients and healthy control groups in terms of age and alcohol parameters in our study (p>0.05), the significant difference was determined between these groups in terms of smoking, cerebrovascular disease history, hypertension, diabetes mellitus and heart disease parameters. Although CT heterozygous genotype of MMP-2 (-1306 C/T) gene variation was determined higher in ischemic stroke patient group compared to healthy controls, the significant difference was not detected between patient and control groups in terms of MMP-2 (-1306 C/T) gene variation genotype distributions. In addition, TT homozygous genotype of MMP-2 (-1306 C/T) gene variation was not observed in ischemic stroke patient group and healthy control group. In conclusion, MMP-2 (-1306 C/T) gene variation was not identified as a genetic risk factor for the development of ischemic stroke in the Thrace population of Turkey. Comprehensive studies with larger populations are needed to determine the relationship between MMP-2 (-1306 C/T) gene variation and the development of ischemic stroke.
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