KÜLTÜR IRKI BESI SIĞIRLARINDA IGF-1 GENINDE TESPIT EDILEN POLIMORFIZMIN KARKAS VE ET KALITE ÖZELLIKLERI ÜZERINE ETKISI
DOI:
https://doi.org/10.5281/zenodo.23101680Keywords:
IGF-1, PCR-RFLP, Polymorphism, Beef cattle, Meat qualityAbstract
Objective: This study was conducted to determine the polymorphisms of the insulin-like growth factor-1 (IGF-1) gene in beef cattle raised in Turkey and to investigate their effects on slaughter and carcass characteristics.
Materials and Methods: The study material consisted of 405 healthy, slaughter-maturity bulls of Hereford, Angus, Charolais, Black Hereford, Brahman, and Limousin breeds, with an average age of 17 months (± 2 months), selected through phenotypic evaluation from a private agricultural enterprise in Şanlıurfa. Characteristics such as backbone area (MLD), back fat thickness, total fat, and fatty acid ratio were determined from the collected meat samples. After DNA isolation from the meat samples, IGF-1 gene polymorphism was detected using the PCR-RFLP method.
Results: In the studied beef cattle herd, the frequency of the B allele (0.600) in the IGF-1 gene was found to be higher than that of the A allele (0.400). The mean MLD area of the IGF-1/SnaBI AA genotype was found to be 61.37 cm2, that of the AB genotype 61.62 cm2, and that of the BB genotype 67.13 cm2. The difference in MLD area between the BB genotype and the AB and AA genotypes was found to be significant (p<0.01).
Conclusion: The relationships observed between the IGF-1/SnaBI polymorphism and carcass and yield characteristics and fatty acid composition suggest that this gene polymorphism can be used as a useful marker in genotypic selection applications.
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